earticle

영어

Alzheimer’s disease(AD) is the most common reason of dementia among people over the age of 65. AD is not yet fully understood about neurodegenerative disease pathology although there have been a lot of studies on AD. Therefore, proteome analysis is very important to understand the changed metabolism of AD brain. In this study, we compared proteome of transgenic (Tg) mice (6 mon Tg, n=16 / 12 mon Tg, n=9) expressing neuronspecific enolase (NSE)-controlled human wild-type tau (NSE-htau23) and control mice (6 mon non-Tg, n=9 / 12 mon non-Tg, n=5) by 2-dimensional electrophoresis (2-DE). As a result, the 27 differentially expressed proteins were identified by ESI-Q-TOF mass spectrometry and LC/MS mass spectrometry. Among the identified proteins, Proteins A, B and C were down-regulated with the progress of AD (p<0.05) and was Protein D up-regulated only in early-stage of AD (p<0.05) these proteins were confirmed by Western blotting. Conclusively, we suggests that these four proteins could help to understand the mechanism associated with neuronal degeneration in AD. And, they are expected to screening of a drug candidate to halt the cascade of disease-associated neurological damage.