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Response of Shewanella oneidensis MR-1 to Long-Term Stress with Ionic Liquid: Comparative Studies on MR-1 And SH-1, an Acclimated Strain

초록

영어

Ionic liquids(ILs) have been known as a green solvent because they are stable solvents. However, it is not clearly known if ILs may possess potential toxic activities when released into the environment. In this study, we investigated the effects of long-term exposure to various ILs on Shewanella oneidensis MR-1, an environmental bacterium. MR-1 was acclimated through repeated exposure to ILs at sublethal concentrations. Among the resulting acclimated strains we selected an [Omim][BF4] acclimated strain for further study. The latter strain was named as SH-1 and compared with MR-1 in various aspects including morphology, growth, and cell surface hydrophobicity (CSH). When grown in a complex medium without IL, SH-1 showed slower growth compared to the MR-1. In the presence of IL growth of SH-1 was slower than that of SH-1 grown without IL. Cell morphology was observed by scanning electron microscopy(SEM) for each strains. Compared to the MR-1, SH-1 showed significantly elongated cell shape. Cell surface hydrophobicity(CSH) analysis was conducted to compare cell surface charges of the two strains. In the absence of ILs there was no significant difference between the two strains.
However, upon exposure to ILs at sublethal concentrations, CSH of SH-1 was rapidly increased while that of MR-1 was not affected.

저자정보

  • Sang-Hyun HAN Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751.
  • Jeong-Min JEON Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751.
  • Kwan Young KIM Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751.
  • Seong Geun KIM Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751.
  • SuYeon JEONG Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751.
  • Choco Michael GOROSPE Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751.
  • Seong Ho HA Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751.
  • Yoon-Mo KOO Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751.
  • Jae-Seong SO Department of Biological Engineering, Colleage of Engineering, Inha University, Incheon, Korea 402-751.

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