earticle

영어

Lignocellulosic biomass is the most favored and valuable compound in bioethanol production. One of the greatest benefits of miscanthus as lignocellulosic biomass is its great biomass yields. Although the miscanthus depends on the species as well as the growth conditions, it can grow up to 4 m tall. However, the lignocellulose is recalcitrant to enzymatic hydrolysis because of its structural complexity. The study, in this sense, was investigated based on characteristics of enzymatic hydrolysis for bioethanol with pretreated biomass using ammonia fiber explosion (AFEX). The AFEX pretreatment of cellulosic feedstock improves its enzymatic digestibility by removing lignin and making the cellulose more accessible to hydrolysis enzymes. As a result, the reasonable conditions were established for the pretreatment process with the temperature set at 150°C, 45 min of reaction time and ammonia concentration of 15% (v/v). In the case of saccharification, it was studied in the basis of enzymatic hydrolysis effects of dosage of enzyme, biomass loading and biomass size. Also, fermentation was carried out based on enzymatic hydrolysis. Ethanol concentration and yield were attained at 37.71 g/L in 24 h and 90.89%, respectively. This study presents not only the overall process but also an efficient bioethanol production from lignocellulosic materials.