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영어

Recently, EM is applied in various fields such as in industry, agriculture and environmental cleanup. Thus, quantitative analysis of EM is necessary as the characteristics and efficacy of EM depends on the distribution ratio of its main genera. Across the study of coexistence mechanisms of EM identifying co-culturing method is obviously necessary. Three strains that are used in this study are Rhodobacter sphaeroides, Lactobacillus plantarum, and Saccharomyces cerevisiae.
Before co-culturing, each strains were grown in van Niel's yeast medium, MRS medium and YM medium respectively. It is possible to co culture of R.sphaeroides and S.cerevisiae. However, overgrowth of L.plantarum caused too high production of lactate, damaging R.sphaeroides by lowering pH. Therefore, to minimize the growth rate of L.plantarum, we have controlled the order and the amount of innoculation under illuminated conditions. The cell concentration of the bacteria was measured by spectrophotometer and cell growth analysis was observed on selective pressured plate counting. The coexistence of these strains were observed by using optical microscope.