earticle

영어

In order to improve bio-ethanol productivity by various cultivation methods in this paper, the culture modes using food wastes, such as batch culture, high-cell-density fermentation, SSF(simultaneous saccharification and fermentation) by fill &draw, continuous culture by fill &draw were performed and their productivities were compared. SSFs by fill &draw were performed by continuous decompression with 1 L evaporator system, and by 10 L bioreactor without decompression. In addition, the continuous cultures by fill &draw mode using SFW(saccharafied food waste) medium were performed by changes of 40% culture broth with intervals of 12 hr(0.03/hr), 6 hr(0.07/hr), 3 hr(0.13/hr). Consequently, productivities of bio-ethanol were 2.52 g/L-hr and 1.30 g/L-hr in batch culture and high-cell-density fermentation, respectively. The productivies of SSF by fill &draw showed 2.24 g/L-hr and 2.03 g/L-hr in continuous decompression with 1 L evaporator and 10 L bioreactor without decompression, respectively. Also, the productivities in continuous culture by fill &draw modes showed 2.02 g/L-hr, 4.07 g/L-hr and 6.25 g/L-hr by medium change with intervals of 12 hr, 6 hr and 3 hrs, respectively. In conclusion, the highest ethanol productivity was obtained in the continuous culture mode by fill &draw with medium change of 40% by 3 hrs(0.13/hr) interval.