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Enhancement of Cellulase Activity of The Hydrolysates by Ultrasonification Pretreatment Process

초록

영어

This study was to increase glucose conversion yield of marine algae, Ulva pertusa Kjellman. Ultrasonification-assisted liquefaction process showed effecting destruction of polysaccharides under following conditions: 120W, 250W, 340W input power for 60min and at a frequency of 20kHz only by using water. The optimal condition of ultrasonification pretreatment was determined as 250W for 60min, having higher sugar yields as 12.2(% w/w), compared to 10.2~11(% w/w) for 700W and 1300W, which indicates that this process could increase the sugar conversion yields up to 10~15% of brown seaweed. Ulva pertusa Kjellman was estimated to contain ca. 6.7% (w/w) of cellulose and 16.8% (w/w) of hemicelluloses, and the hydrolysates from this process was also easily digested only 5FPU/ml of cellulase. About 97% of cellulose was converted into glucose under this condition, which implied that this ultrasonification-assisted liquefaction could be employed to effectively hydrolyze celluloses of marine algae. Consequently, the improvement is primarily attributed to the ultrasonification process for the enhanced enzyme activity of cellulose hydrolysis.

저자정보

  • Woon Yong CHOI Dept. of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701.
  • Sung Ho OH Dept. of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701.
  • Yong Chang SEO Dept. of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701.
  • Ji Seon KIM Dept. of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701.
  • Do Hyung KANG Korea Ocean Research & Development Institute, Ansan P.O. Box 29, Seoul 426-744.
  • Hyeon Yong LEE Dept. of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701.

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