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Optimization of culture conditions for mass production of several microalgae using response surface method

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Culture conditions for mass production of Chlorella schroteri, Dunaliella salina and Dunaliella sp. were optimized using response surface method (RSM). A central composite design, which was developed to facilitate the RSM, was applied to investigate the effects of initial pH, nitrogen sources and phosphate concentration on the cultivation of microalgae.
The optimum growth conditions estimated from the design were: Chlorella schroteri (pH 7.2, ammonium 17mM, phosphate 1.2mM), Dunaliella salina (pH 7, nitrate 3.3mM, phosphate 0.0375mM) and Dunaliella sp. (pH 8, nitrate 3.7mM, phosphate 0.17mM). Culturing of the microalgae with the optimized conditions confirmed that the maximum growth rates were attained from the parameters. The specific growth rates(μmax) of Chlorella schroteri, Dunaliella salina and Dunaliella sp. were 0.99, 3.45 and 4.33/day, respectively. The biomass productivity of Chlorella schroteri, Dunaliella salina and Dunaliella sp. were 0.22, 0.46 and 0.23g dry cell weight/L/day, respectively.

저자정보

  • Jang Min PARK Dept. of Environmental Engineering, Pioneer Research Center for Controlling of Harmful Algal Bloom, Chosun University, Gwangju 501-759, Korea.
  • Si Wouk KIM Dept. of Environmental Engineering, Pioneer Research Center for Controlling of Harmful Algal Bloom, Chosun University, Gwangju 501-759, Korea.

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