원문정보
초록
영어
Several agar-degrading bacteria were isolated from gut of spiny turban shells. These bacteria were examined by Lugol’s iodine and identified as extracellular agarase-producing bacteria. Among them, one species made clear depressions around colonies. Agar-degrading ability of this bacterium was examined by measuring viscosity-change of the inoculated medium. The viscosity of the inoculated medium was decreased by more than 60% after 20 hour-cultivation while pure medium did not show any decline of viscosity. The sequence of 16s rRNA of the bacterium was analyzed and a comparative analysis of the 16s rRNA sequence was performed by BLASTN with Genebank data base. The result indicated that it might be one species of the genus, Pseudoalteromonas. In addition, a homology-comparison-study of relative strains was performed, and as a result, the bacterium was identified as one of Pseudoalteromonas nigrifaciens strains.
Experiments, such as assay of enzyme activity, enzyme identification are being performed for further study.