earticle

영어

Reactive oxygen species (ROS) have been shown to cause DNA damage, denaturation of protein, loss activity on anti-oxidative enzyme and lipid peroxidation. Thus, ROS are associated with tissue damage and the prime contributing factors for inflammation, diabetes, aging, cancer, hypertension and arteriosclerosis. Some mushrooms, including Phellinus linteus, commonly produce a bundle of yellow antioxidant pigments that are composed of hispidin derivatives and polyphenols. Hispidin, metabolite of Phellinus linteus, is a potent antioxidant and inhibitor of protein kinase C (PKC). In this study, it was investigated whether hispidin would protect MIN6N pancreatic β-cells from oxidative stress by hydrogen peroxide. Treatment of MIN6N β-cells with 0.5mM hydrogen peroxide for 4 hours could cause a significant cell viability loss and an increase in apoptotic cells. Pre-treatment of MIN6N β-cells with hispidin for 24 hours reduced cell viability loss and decrease in apoptotic cells.
In addition, 70μM hispidin significantly scavenged the intracellular ROS and inhibited apoptosis induced by hydrogen peroxide. In addition, the generation of thiobarbituric acid reactive substance (TBARS) was inhibited in the presence of hispidin in a dose-dependent manner. Also, 70μM hispidin significantly increased insulin secretion. These results suggested that hispidin might reduce oxidative stress damage of MIN6N β-cells by hydrogen peroxide.
Hispidin may be effective in preventing MIN6N β-cells from the toxic action of reactive oxygen species in diabetes.