원문정보
초록
영어
As a part of search for antioxidants from marine organisms, antioxidant activities of two seagrasses Zostera japonica and Z. asiatica were investigated. Each of them was extracted two times with acetone-CH2Cl2(1:1) and MeOH in turn. Crude extracts were combined for each sample. Each of the combined crude extracts was partitioned between CH2Cl2 and water. The organic layer was further partitioned between 85% aq MeOH and n-hexane, and the aqueous layer was fractionated with n-BuOH and H2O, successively. The protective effect of each fraction from Z. japonica and Z. asiatica on H2O2-induced oxidative stress was examined in Raw 264.7 cells. The levels of intracellular ROS and GSH were measured using 2',7'-dichlorofluorescin diacetate and monobromobimane as fluorescence probe, respectively. All solvent fractions including their crude extracts not only significantly decreased levels of intracellular ROS in a dose-dependent manner but also increased intracellular GSH level. Moreover, they exhibited a significant protective effect on membrane lipid peroxidation in TBARS assay system. These results suggest that potential natural antioxidants related to oxidative stress could be isolated from Z. japonica and Z. asiatica