원문정보
초록
영어
We synthesized a hydroquinone glucoside (HG) as a potential skin whitening agent using Leuconostoc mesenteroides dextransucrase with hydroquinone (HQ) as an acceptor and sucrose as a donor. The product was purified using butanol partitioning and silica-gel column chromatography. The structure of the purified HG was determined by NMR. HG was identified as 4-hydroxyphenyl-α-D-glucopyranoside.
The optimum condition of HG synthesis, determined using a response surface methodology, was 450 mM HQ, 215 mM sucrose, and 0.55 U/mL dextransucrase; the final HG produced was 544 mg/L HG potently inhibited oxidation. The IC50 of diphenylpicryl-hydrazyl scavenging activity was 3.85 mM indicating a higher anti-oxidant activity compared to β-arbutin (IC50 = 6.04 mM). HG-mediated inhibition of lipid peroxidation was 3.51% that of HQ (100%) and much higher than that of β-arbutin (0.81% of HQ). In addition the IC50 value of nitrite scavenging activity was 14.76 mM showing a superior scavenging activity to that of β-arbutin (IC50 = 27.09 mM)