earticle

영어

The yeast pichia pastoris has been well known as a highly successful system for the production of various recombinant heterologous proteins. To improve scFv production in pichia pastoris, parameters such as cultivation temperature, initial pH of medium and cell density were examined. Investigation of culture conditions revealed that the maximal cell growth and functional scFv expression were achieved at pH 6.0-7.0 and at 20 ℃ which is relatively low temperature. The N-terminal sequence of expressed anti-BNP scFv was analyzed and two Ste13 sites were not cut out at all unlike our expectation. However the function or activity of anti-BNP scFv was not affected significantly. The optimized culture conditions determined through this study will be used from the efficient production of anti-BNP scFv.