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The role of phosphomannomutase in Streptomyces coelicolor

원문정보

Yung-Hun YANG, Eunjung SONG, Jinu KIM, Sanghyun LEE, Hyung Joo KIM, Byung-Gee KIM

한국생물공학회 한국생물공학회 학술대회 2010 추계학술대회 및 국제심포지움 2010.10 pp.193-193
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초록

영어

Phosphomannomutase (ManB), whose main function is the conversion of mannose-6-phosphate to mannose-1-phosphate, is involved in biosynthesis of GDP-mannose for numerous processes such as synthesis of structural carbohydrates, production of alginates and ascorbic acid, and post-translational modification of proteins in prokaryotes and eukaryotes. ManB from Streptomyces coelicolor was shown to have both phosphomannomutase and phosphoglucomutase activities. Deletion of manB in S. coelicolor caused a dramatic increase in actinorhodin (ACT) production in the low-glucose Difco nutrient (DN) medium and slight decrease of sporulation in complex medium (R5-). Experiments involving complementation of the manB deletion showed that increased ACT production in DN media was due to blockage of phosphomannomutase activity rather than phosphoglucomutase activity. These results provide useful information for the role of phosphomannomutase in S. coelicolor on antibiotic production and sporulation.

키워드

저자정보

  • Yung-Hun YANG Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul 143-701, Korea.
  • Eunjung SONG School of Chemical and Biological Engineering, Seoul National University.
  • Jinu KIM School of Chemical and Biological Engineering, Seoul National University.
  • Sanghyun LEE Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul 143-701, Korea.
  • Hyung Joo KIM Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul 143-701, Korea.
  • Byung-Gee KIM School of Chemical and Biological Engineering, Seoul National University.

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