earticle

영어

Anthracycline antibiotics including doxorubicin and darubicin are clinically important anticancer agents. It has been reported that these drugs intercalate into DNA and interact with topoisomerase II-DNA complex. Epirubicin is a semisynthetic analogue of doxorubicin displaying reduced cardiotoxic and equal antitumor property when compared with doxorubicin. In order to enhance epirubicin production in S. venezuelae system, we assayed four glycosyltransferases and eight 4-ketoreductases from various Streptomyces species. The results of this assay suggested that glycosyltransferase AknS and 4-ketoreeductase AvrE are the best enzymes among the glycosyltransferases and 4-ketoreeductases tested.