원문정보
초록
영어
Gene therapy is considered as a potential remedy for treating diseases.
Therefore, safe and efficient gene delivery materials have been investigated. Gene delivery vectors are categorized into two groups: viral and non-viral. Though viral vectors are most effective, safety concerns are limitations on clinical cure. However, non-viral vectors have improved safety and manufacturing capacity. Here, we report the recombinant mussel adhesive protein (MAP) as a synthetic DNA delivery vehicle. Recombinant MAPs are biocompatible, biodegradable proteins for underwater adhesion. Because of the plenty of basic amino acid composition, they were considered as a possible carrier of DNA.
After DNA binding assay, mouse NIH3T3 cells were transfected with pEGFP by MAP. The transfection efficiency was compared with LipofectamineTM 2000, the widespread transfection agent. Optimized transfection condition will achieve better capability in transferring DNA into model cell line.