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영어

Streptomyces peucetius industrial mutant was recursively mutated to be able to overproduce doxorubicin (DXR) than S. peucetius ATCC27952.
Previously we identified wblA, a global antibiotic down-regulator, using a Streptomyces interspecies DNA microarray analysis. Also we confirmed that disruption of wblA ortholog in S. peucetius industrial mutant resulted in an approximately 70% increase in DXR productivity than those of S. peucetius industrial mutant strain. Deletion of DXR gene cluster from the chromosomes of S. peucetius industrial mutant and wblA-disrupted S. peucetius OIM strains enable to verify possibility of heterologous expression of polyketide synthase (PKS) gene cluster as a host. A subset of S. coelicolor actinorhodin (act) biosynthetic genes (act Ⅰ-orf1, actⅠ-orf2, actⅠ-orf3, actⅢ, actⅦ, actⅣ) for aloesaponarinⅡ, a highly fluorescence anthraquinone polykeitde derived from one acetyl-CoA and seven malonyl-CoA extender units, and actinorhodin cluster activator gene (actⅡ-orf4) was successfully cloned to express in both S. peucetius OIM△DXR and S. peucetius △wblA△DXR strains.