earticle

영어

L-isoleucine, one of branched chain amino acids, has been considered an industrially valuable product since it is employed as a component of pharmaceuticals, cosmetics and dietary products as well as animal feed additives. Escherichia coli, the host organism of this study, tightly regulates L-isoleucine biosynthetic pathway by operating complex regulatory mechanisms, including feedback inhibitions and transcriptional regulations. In this study, we removed all the negative regulations that hamper the production of L-isoleucine, and overexpressed the genes encoding enzymatic reactions and exporters in order to improve the host strain’s L-isoleucine-producing capability. The approaches described in this study are a good example of rationally engineering microorganisms for the enhanced production of L-isoleucine, and they can also be generally applicable to development of strains for the efficient production of other valuable bioproducts. [This work was supported by the Korean Systems Biology Research Project (20100002164) and WCU (World Class University) program (R322009000101420) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea.]