원문정보
초록
영어
Heterologous protein production in microorganisms has been gaining momentum as tools and techniques for genetic manipulation become more sophisticated. Traditionally, heterologous proteins have been produced in small model multi-cellular organisms. However, due to the time and resources used to care for these ‘factories’, alternate and simpler systems are sought. Yeasts have been the preferred unicellular system for eukaryotic studies and the methylotrophic yeast Pichia pastoris has gained much attention as a host, particularly for proteins with therapeutic values. Here, the genome-scale metabolic model is presented and the analysis of P. pastoris capabilities in producing proteins is investigated. The production of two proteins, human serum albumin (HSA) and human superoxide dismutase (hSOD), were analyzed and metabolic engineering targets were suggested to improve their production. Using genome-scale metabolic model, potential phenotypes can be screen in a high-throughput manner, accelerating the development of strategies for engineering improved strains. [This work was supported by the Korean Systems Biology Research Project (20100002164) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea, by the Austrian Science Fund (FWF), project I37-B03, and the Austrian Research Promotion Agency (program FHplus)]