earticle

영어

Osteoarthritis (OA) is the most common rheumatic pathology. A major objective for OA research is development of early diagnostic strategies which include a proteomic technology. Synovial fluid (SF) is a logical potential compartment for OA biomarkers because it is derived directly from the diseased site and the systemic circulation. To enrich relatively lower-abundant proteins in the SF sample, the efficient and reliable preparation of SF samples is very critical in proteome analysis. In this study, we evaluated the pattern of SF proteins on 2 DE-PAGE as a function of sample enrichment and protein loading. Removal of albumin and IgGs and hyaluronidase treatment improve the detection of protein spots on 2DE-PAGE.