earticle

영어

It has been demonstrated that the approach of enzyme coatings on various nanomaterials is successful in stabilizing the enzyme activity in an unprecedented way [1,2,3]. For example, the trypsin coatings on electrospun polystyrene-poly(styrene-co-maleic anhydride)(PS-PSMA) nanofibers showed no decrease of enzyme activity under recycled uses and rigorous shaking for one year [3]. In the present work, trypsin was covalently attached or coated onto PS-PSMA nanofibers with varied concentrations of maleic anhydride group, which enables an easy conjugation of enzymes onto nanofibers. The concentration of maleic anhydride group correlated well with the enzyme loading and activity of covalently-attached trypsin. Trypsin-coated nanofibers, prepared via covalent attachment and follow-up enzyme crosslinking, resulted in highly stable nanobiocatalytic systems, irrespective of added PSMA amounts. The variation of PSMA amounts enables the facile control of various properties of trypsin-coated nanofibers, such as enzyme loading, activity, structure, and mass transfer limitation. The property control of highly stable enzyme coatings would provide a powerful tool for more successful applications of enzyme coatings in various fields.