원문정보
초록
영어
Lignin is an abundant renewable polymer that is a byproduct of biofuel synthesis. To use lignin as feedstock for chemicals and polymers, it can be fragmented to p-coumaric acid and other aromatic fragments Phenolic acid decarboxylase (PDC) catalyzes the non-oxidative decarboxylation of p-coumaric acid (pCA) to p-hydroxystyrene (pHS), which is a polymer monomer PDC from Lactobacillus plantarum is a well known enzyme and we used it as a template to search sequence databases for other, potentially more active, PDCs. The retrieved sequences were arranged in a phyologenetic tree and representativesequences from each of the five groups were cloned and over-expressed in PDC from Bacilllus amylolichefaciens (BaPDC) had 1.5-fold higher specific activity for p-coumaric acid than PDC from Lactobacillus plantarum and a 10-fold higher kcat/Km The initial rate of BaPDC-catalyzed formation of p-hydroxystyrene was 1.44 mmol/min/g DCW, but slowed due to product inhibition (Ki = 0.5 mM). Using a compatible organic solvent we can extract high concentration of the pHS seperated from water in biphasic reaction.