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효소공학

Cloning of an Endo-1,4-β-Xylanase C from Phaenerochaete chrysosporium, and Its Expression in Pichia pastoris

원문정보

Seung-Moon PARK, Nguyen Duc HUY, Ae-Young MO, Yu-Lim SON, Han-Sung PARK, Dae-Hyuk KIM

한국생물공학회 한국생물공학회 학술대회 2010 추계학술대회 및 국제심포지움 2010.10 pp.179-179
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

An enzyme of the glycosidase hydrolase family 10 endo-1,4-β-xylanase C from Phaenerocheate chrysosporium was cloned into the pPICZ and pPICZα vectors and expressed in Pichia pastoris under the control of the methanol inducible alcohol oxidase I (AOXI) promoter. Enzyme assay indicated that both intrinsic signal peptide and P. pastoris α-factor secretion signal peptide were efficient in mediating Xylanase C secretion. Xylanase C activity reached 2.5 unit/ml in medium culture after induction for three days with 1% methanol. The enzyme activity in this study is four to five times higher than previously reported Xylanase expression in Aspergillus niger. The optimal temperature and pH the enzyme are 70OC and 4.5, respectively.

키워드

저자정보

  • Seung-Moon PARK Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Jeonju, 561-756.
  • Nguyen Duc HUY Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Jeonju, 561-756.
  • Ae-Young MO Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Jeonju, 561-756.
  • Yu-Lim SON Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Jeonju, 561-756.
  • Han-Sung PARK Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Jeonju, 561-756.
  • Dae-Hyuk KIM Division of Biological Sciences, College of Natural Sciences, Chonbuk National University, Jeonju, 561-756.

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