earticle

영어

Highly stable and active nanoscale enzyme reactor (NER) [1] has been developed by adsorbing and crosslinking glucose oxidase (GOx) in polyaniline nanofibers (PANFs). PANFs, prepared by oxidative polymerization with ammonium persulfate as an oxidant [2], contains nanometer-scale pores that enable the NER approach. The simple addition of enzyme crosslinking step resulted in highly stable and active enzyme systems. For example, the activities of adsorbed GOx (ADS) and NER were 0.040 and 0.382 (A500/min) per 0.1 mg PANFs, respectively. This 9.6-fold increase of enzyme activity can be explained by the improved enzyme loading of NER because the enzyme crosslinking can effectively prevent the enzyme leaching from PANFs. Stability-wise, ADS retained only 50% of initial activity after 5 day incubation at room temperature while NER maintained more than 90% of initial activity in the same condition for two months. To demonstrate the feasible application of highly stable and active NERs in conductive PANFs, the operation of biofuel cells (BFCs) were tested with enzyme anodes based on ADS and NER. The detailed result will be given in the presentation.