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Cellulose-based materials have many potential applications in biomedical fields, because of their biocompatibility and biodegradability. Carbon nanotubes (CNTs) have been reported as ideal electrode materials due to their high electrical conductivity and large surface area. Recently, it was also reported that CNTs facilitate the direct electron transfer (DET) from redox enzymes to electrodes.1 DET between glucose oxidase (GOx) and electrodes is essential for GOx-based biosensors, biofuel cells, and bioelectronic devices, although GOx generally requires mediators for electrical communications.2 In this work, cellulose-CNT composites were prepared by vacuum filtration of CNTs and coagulation of cellulose dissolved in ionic liquid. Furthermore, GOx was immobilized on cellulose-CNT composite. Cyclic voltammograms of cellulose-CNT-GOx electrodes showed a pair of well-defined peaks. The formal redox potential peak was -460 mV, which agreed well with that of FAD/FADH2. This result clearly shows that the DET between the GOx and the cellulose-CNT electrode was achieved. It was also found that the GOx immobilized on the electrode retained catalytic activity for the oxidation of glucose.