원문정보
초록
영어
Agar is a structural polysaccharide found in cell walls of red algae and is generally considered to be a mixture of agarose and agaropectin. Enzymatic hydrolysis of sulfate groups in agaropectin or agar simplifies the production process of high-quality or low sulfate-content agarose. In this work, arylsulfatase gene (astA, 984 bp ORF) from Pseudoalteromonas carrageenovora was cell-surface displayed in S. cerevisiae and applied to desulfatate agar. The arylsulfatase gene was fused in frame with GAL1 promoter and AGA2 gene. To prepare the desulfatated agar, the commercial agar was treated with arylsulfatase displayed S. cerevisiae cell. The sulfate content in agar was decreased to 0.2% as a yeast biocatalyst concentration-dependent manner.
Electrophoresis of λDNA HindⅢ marker and 1 kb DNA ladder with the arylsulfatase treated agar and commercial agarose showed the similar pattern of DNA migration and resolution. In addition, the gel strength of arylsulfatase treated agar and commercial agarose were compared.