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Development and characterization of a mutated soluble epoxide hydrolase of Denio rerio

원문정보

Eun Yeol LEE, Sung Hee CHOI, Hee Sook KIM

한국생물공학회 한국생물공학회 학술대회 2010 추계학술대회 및 국제심포지움 2010.10 pp.177-177
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

A mutated soluble epoxide hydrolase (sEH) from Danio rerio was developed and characterized. We obtained a mutated D. rerio sEH possessing five-point mutations. The catalytically important amino acids of Asp331, Tyr379, Tyr460, Asp496, and His524 were maintained, indicating that the mutated sEH could possess hydrolytic activities. Kinetic characteristics of the mutated sEH were analyzed, and the activity was proved to be enhanced. Enantiopure 99 %ee styrene oxide was prepared by using the mutated D. rerio sEH as the biocatalyst.

키워드

저자정보

  • Eun Yeol LEE Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701.
  • Sung Hee CHOI Department of Food Science and Biotechnology, Kyungsung University, Busan 608-736.
  • Hee Sook KIM Department of Food Science and Biotechnology, Kyungsung University, Busan 608-736.

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자료제공 : 네이버학술정보

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