earticle

영어

Candida antartica lipase B (CALB) has been widely applied in industrial field such as pharmaceutical, food processing. For the production of biodiesel using lipase, reaction condition needs to operate at high temperature because both triglyceride and fatty acid methyl ester have high viscosity in mild temperature.[1] Novel route for immobilized autosilcificated CALB-R1, which is fusion protein between CALB and R1 silaffin peptide, was suggested for improvement of thermostability. Target enzyme was entrapped as over 90% entrapment efficiency in produced silica gel.[2] Additionally, this result shows that thermostability of CALB-R1 was not improved critically and influenced by environmental pH value. pH value was main factor for biosilicification because this reaction was carried out between protein pI value and pH of biosilicification. CALB-R1 was adsorbed in VPOC 1600 (commercial support material) and immobilized CALB-R1 was successfully coated with silica film using autosilicification. Immobilized autosilicificated CALB-R1 showed be much improved thermal stability owing to restricted conformational variation by novel entrapment immobilization method.