earticle

영어

The demand for biopolymers-based materials has recently gained worldwide attention in the biomedical fields such as tissue engineering, drug delivery systems, dialysis membranes, and biosensors owing to their inherent biocompatibility and biodegradability. However, the insolubility of unmodified native biopolymers in most organic solvents has limited the applications of biopolymer-based materials and composites. Ionic liquids have a great potential to dissolve biopolymers and develop biopolymer-based materials, because of their synthetic flexibility by changing the combinations of cation and anion, and green solvent properties such as non-volatility, non-flammability and recyclability.
In this work, biocompatible ionic liquid, [Emim][acetate], was used to encapsulate lipase into various biopolymer-based composite beads.
Cellulose, cellulose/chitosan, cellulose/agarose, and cellulose/agar beads containing lipase from Candida rugosa were prepared by co-dissolution of biopolymers and lipase in [Emim][acetate] followed by reconstitution with distilled water. Cellulose/chitosan was the most efficient composite to encapsulate and retain activity of lipase.