원문정보
초록
영어
Tautomycetin (TMC), a secondary metabolite produced by Streptomyces sp. CK4412, is a novel activated T cell-specific immunosuppressive compound with an ester bond linkage between a terminal cyclic anhydride moiety and a linear polyketide chain bearing an unusual terminal alkene. Previously, we isolated and characterized the entire TMC biosynthetic gene cluster from Streptomyces sp. CK4412 [1].
Within the TMC biosynthetic gene cluster, we found two putative pathway-specific genes, tmcN and tmcT. Both tmcN and tmcT were identified as positive regulators by targeted gene disruption as well as in silico sequence comparison [2]. Here, we isolated, identified, and disrupted a wblA ortholog gene (named wblA-tmc) from Streptomyces sp. CK4412, which was previously reported as a strong global down-regulator of antibiotic biosynthesis in various Streptomycetes speices. Moreover, we added additional tmcN or tmcT into ΔtmcT or Δ tmcN mutants and also disrupted wblA-tmc gene from each of ΔtmcN or ΔtmcT to understand how these regulatory genes be related in TMC biosynthetic gene cluster of Streptomyces sp. CK4412. The detailed results will be further discussed.