원문정보
초록
영어
The effects of raw glycerol on 1,3-propanediol (1,3-PDO) production were investigated with various microorganisms. Klebsiella strains were generally resistant to the tested 4 types of raw glycerol while the growth of Clostridium strains was variably inhibited depending on the type of raw glycerol. Fed-batch cultures with suspended cells of Klebsiella pneumoniae DSM 4799 revealed that 1,3-PDO production was more effective when utilizing raw glycerol than pure glycerol. In addition, more than 80 g/L of 1,3-PDO was produced using raw glycerol; this is the highest 1,3-PDO concentration reported thus far for K. pneumoniae using raw glycerol. Repeated fed-batch fermentation with cell immobilization in a fixed-bed reactor (FBR) was performed to enhance 1,3-PDO production. Production of 1,3-PDO increased with the cycle number due to the successful cell immobilization. Continuous fermentation with immobilized cells was also performed to investigate the effect of dilution rate and glycerol concentration on 1,3-PDO production.