원문정보
초록
영어
The optimal conditions for the mass production of carboxymethylcellulase (CMCase) of Bacillus amyloliquefaciens by a recombinant E. coli JM109/DL-3 were established in this study using an orthogonal array method. Rice bran and tryptone were found to be the best carbon and nitrogen sources for the production of CMCase by E. coli JM109/DL-3.
The optimal concentrations of rice bran and tryptone and the initial pH for the production of CMCase were 50.0, 2.5 g/L, and 7.5, respectively.
The optimal concentrations of K2HPO4, NaCl, MgSO4·7H2O, and (NH4)2SO4 in the medium for the production of CMCase by E. coli JM109/DL-3 were 5.0, 1.00, 0.40, and 0.60 g/L. The optimal agitation speed and aeration for cell growth of E. coli JM109/DL-3 in 7 L bioreactors were 500 rpm and 1.0 vvm, whereas those for the production of CMCase were 400 rpm and 1.0 vvm. The optimal inner pressure for cell growth of E. coli JM109/DL-3 in 100 L bioreactors was found to be 0.8 kgf/cm2, whereas that for the production of CMCase was 0.6 kgf/cm2. The production of CMCase with an inner pressure of 0.6 kgf/cm2 was 1.36 times higher than that without an inner pressure.