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포스터 발표 : 미생물공학

Screening for Antimicrobial Activity against Diarrhoeal Pathogens in the Growing Pig

초록

영어

A wide range of enteropathogens cause serious diarrhoeal diseases in fattening piglets and account for food-related infections in humans. The objective of this study was to screen beneficial bacterial strains from the gastrointestinal tract of animal sources and lactic acid bacteria(LAB) for antimicrobial activity against diverse hazardous pathogens associated with pig growth period. In this work, 3 strains from porcine gut compartment and 6 strains from KCCM were cultured in MRS medium at 37℃ for 48hr. Using Kirby-Bauer method, 9 well-characterized strains belonging to Lactobacillus were studied for inhibition against Escherichia coli O-157, Escherichia coli K88, Staphylococcus aureus, Salmonella enteritidis, as well as Salmonella typhimurium. Potential antimicrobial strains were analyzed by studying their cell-free supernatants without pH neutralization. E. coli O-157 was effectively inhibited by L. acidophilus, L. rhamnosus, L. casei and L. reuteri LA26-1 strains. E. coli K88 and S. enteritidis were inhibited by L. acidophilus, L. rhamnosus, and L. plantarum. The findings of this study provide a basis to encourage feeding studies to evaluate the antimicrobial potential of promising strains in pig production.

저자정보

  • SunA YOON National Research Lab of Skin-Bioactive Materials Lab., Dept. of Biological Engineering, Inha University, Incheon, Korea.
  • Ji Hyuk YOO National Research Lab of Skin-Bioactive Materials Lab., Dept. of Biological Engineering, Inha University, Incheon, Korea.
  • Dung Hoang NGUYEN National Research Lab of Skin-Bioactive Materials Lab., Dept. of Biological Engineering, Inha University, Incheon, Korea.
  • Jimin UM National Research Lab of Skin-Bioactive Materials Lab., Dept. of Biological Engineering, Inha University, Incheon, Korea.
  • Wooyoung CHOI National Research Lab of Skin-Bioactive Materials Lab., Dept. of Biological Engineering, Inha University, Incheon, Korea.
  • Man Ki SON National Research Lab of Skin-Bioactive Materials Lab., Dept. of Biological Engineering, Inha University, Incheon, Korea.
  • Sang-Joo PARK National Research Lab of Skin-Bioactive Materials Lab., Dept. of Biological Engineering, Inha University, Incheon, Korea.
  • Eun-Ki KIM National Research Lab of Skin-Bioactive Materials Lab., Dept. of Biological Engineering, Inha University, Incheon, Korea.

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