원문정보
초록
영어
To elucidate the function of an unknown regulator in Streptomyces, differences in phenotype and antibiotic production have been compared between a deletion mutant and wild type strain. However, these differences are easily hidden by complex media. Thus, we developed a multi-well method containing different nutrients along with bromothymol blue to determine the specific media conditions which can reveal such differences easily. By comparing the growth of wild type and mutant strains on the screened nutrient conditions, we were able to measure growth, organic acid production, and antibiotic production to elucidate the function of unknown regulator. As a result, a member of the MarR-like regulator family, SCO5405 (AbsC), was newly characterized. Deletion of SCO5405 increased the pH of the culture broth due to decreased production of organic acids such as pyruvate and α-ketoglutarate and increased extracellular ACT production in minimal media containing glucose and alanine.
Therefore, this method could be a high-throughput method for the characterization of unknown regulators.