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In a previous study, we have isolated 108 strains of Lactobacillus spp. from human. In this study, 22 strains showing antagonistic activity against avian pathogens Salmonella enteritidis and S. gallinarum were selected among them. One strain out of 22 strains which had high-leve of antimicrobial activity was identified as L. paracasei (KLB58) by 16S rRNA gene sequencing. The activity was heat stable and proteincaeous.
The mode of action was shown to be bacteriostatic, and the antimicrobial substances were produced during the early stationary phase. In order to assess potential usage as probiotics for poultry KLB58 strain was tested for its ability to prevent Salmonella gallinarum infection in chickens. Chickens challenged with S. gallinarum showed markedly increased death rate compared with the control group.
However, the death rates were significantly lower in chickens (13.3%) supplemented with KLB58 than in those (40%) not supplemented (P<0.05). Therefore, we suggest that L. paracasei KLB58 has potential probiotic effect for chickens. Further studies are underway to characterize the antimicrobial substance.