earticle

영어

Porcine epidemic diarrhea virus (PEDV) is a causative agent for acute enteritis and lethal watery diarrhea in pigs, particularly in neonates, with a high mortality rate. Outbreaks of the disease were reported from numerous European countries as well as Korea, China and Japan.
PEDV, a member of the family Coronaviridae, is an enveloped virus possessing a single-stranded positive-sense approximately 28 kb RNA genome. A cell culture system for PEDV propagation was developed and successfully used for virus isolation. However, molecular technologies have been commonly exploited for rapid detection of viruses before detection of virus propagation. Efficient molecular methods for high throughput detection of diverse types of PEDV are not feasible yet. Among several genes of PEDV genome, genes coding for the M protein (a structural membrane glycoprotein, which plays an important role in the assembly process) and the S surface glycoprotein (harbors the specific host cell receptor binding sites) were commonly used for the identification and immunological purpose. In this study, we analyzed diversity of gene sequences of M and S proteins available in public databases and selected gene regions with high variability which could be selected as candidates for design of specific probes.