원문정보
초록
영어
2,3-Butanediol(2,3-BDO) is a key starting material for manufacture of bulk chemicals such as methyl ethyl ketone and 1,3-butadien. It also has potential applications in the manufacture of printing ink, perfumes and explosive. Microbial production of 2,3-BDO by using Klebsiella pneumoniae was first investigated in 1906. Klebsiella pneumoniae is a Gram-negative, non-motile, encapsulated, lactose fermenting, facultative anaerobic, rod shaped bacterium found in the normal flora of the mouth, skin, and intestines. In this study, in order to enhanced 2,3-BDO production, budC gene coding for butanediol dehydrogenase was over-expressed and ldhA gene coding for lactate dehydrogenase disrupted by inserting a tetracycline resistance marker in Klebsiella pneumoniae KTCT2242. The fermentation results of both the parent and mutant strain suggested that over-expression of budC and inactivation of ldhA resulted in an improved 2,3-BDO production.