earticle

영어

Fatty acid biosynthesis in E. coli is dedicated with conversion of acetyl-CoA to malonyl-CoA with acetyl-CoA carboxylase (ACC) which catalyze the ATP-dependent first step. The fatty acid synthase converts malonyl-CoA using NADPH as a source of reducing equivalence to an acyl carrier protein (ACP). In fatty acid biosynthesis, fabH, fabB and fabF genes constitute an enzyme, β-ketoacyl acyl carrier protein synthase III that catalyze the first committed step in fatty acid biosynthesis(ATP-dependent formation of malonyl-CoA from acetyl-CoA and bicarbonate). fabG is a gene for β-ketoacyl acyl carrier protein reductase and fabI is a gene for NADH-dependent enoyl-acyl carrier protein reductase that is related to the completion of fatty acid elongation. In this study, a vector system was constructed to increase short chain fatty acid (C3-C6) content in E. coli. E. coli W3110 was used for the preparation of genes and E. coli BL21 (DE3) was used as a host cell for the expression of target genes, fabH, fabB, fabF, fabG and fabI.