earticle

영어

Chinese hamster ovary (CHO) cells are the most widely used mammalian host for the commercial production of recombinant proteins. Recently, various strategies have focused on increasing specific productivity in CHO cell culture. One of the most important mechanisms of regulating of gene expression is the modulation of histones via acetylation. These histone hyperacetylation and enhancement of the expressed mRNA transcription level of target protein were regulated by histone deacetylase inhibitor (HDACi). Effects of HDACi on the enhanced production of recombinant proteins expressed in CHO cells have been reported. To determine the effects of HDACi on the production of recombinant albumin-erythropoietin (Alb-EPO) fusion protein, various inhibitors were added to the cell cultures in exponential growth phase. Among the inhibitors tested, sodium propionate and sodium butyrate were found to be efficient. As these compounds induced cell death, it was hypothesized that HDACi actually induced cell death via apoptosis rather than necrosis. Compared to control cultures without sodium butyrate, the culture with 1 mM sodium butyrate resulted in a 1.7-fold increase in Alb-EPO concentration on day 6. In conclusion, it was found that HDACi enhanced the production of Alb-EPO but also the apoptosis of recombinant CHO cells.