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초록
영어
A cryptic plasmid, pMBLR00, was isolated from Leuconostoc mesenteroides subsp. Mesenteroides KCTC 3733. Complete sequencing of pMBLR00 revealed a 4163 bp element with G+C content of 37% harboring three open reading frames (ORFs A, B and C). The ORF A gene product has 98% amino acid identity with the Rep protein of a rolling-circle replication (RCR) plasmid pYS18, isolated from Lactobacillus sakei YSI8. Southern hybridization and nuclease treatment demonstrated that pMBLR00 contains a single-stranded intermediate, confirming that pMBLR00 replicates via RCR mechanism.
The ORF B gene product is homologous to mobilization proteins encoded by plasmids isolated from Leuconostoc and Lactobacillus species. An E. coli-Leuconostoc shuttle vector pMBLR01 was constructed based on pMBLR00. The shuttle vector pMBLR01 was stable in Leuconostoc citreum.