earticle

영어

Acetone/pentane precipitation is a simple, efficient method for pre-purifying paclitaxel extracted from plant cell cultures. However, the acetone/pentane precipitation process has been inherently problematic due to the lengthy precipitation time that is required. An improved acetone/pentane precipitation process could significantly reduce the precipitation time by increasing the surface area per working volume (S/V) of the reacting solution through the addition of an anion exchange resin (Amberlite IRA400) or glass beads. Most of the paclitaxel (>95%) could be obtained after about 24 h of acetone/pentane precipitation by increasing the surface area of reactor. Purity increased with increasing acetone/pentane precipitation time up to 24 h to about 80%, after which it showed little change. The size of paclitaxel precipitates was measured with the electronic microscope, it was observed that the use of an exchange resin had an effect on inhibition of precipitate growth compared to prepared particles without exchange resin. In addition, the amorphous forms of paclitaxel were prepared using acetone/pentane precipitation method. This improved pre-purification process serves to minimize solvent usage and the size and complexity of the high performance liquid chromatography operation required for paclitaxel purification.