원문정보
초록
영어
Exopolysaccharides(EPS) from Aureobasidium pullulans are used for health foods and cosmetics. In order to better exploit EPS production from A. pullulans, medium composition was optimized using stastical methods. And, effect of each nutrient of basal medium was investigated by preliminary studies (one-factor-at-a-time). Maximum growth and EPS production were observed at various initial pH 3-5, while growth and EPS production were also very low at pH 2 and pH 7. Among various nitrogen sources (Ashbya gossypii extract, beef extract, corn steep liquor, corn steep, powder, malt extract, soytone peptone and yeast extract) tested, the highest mycelial growth and EPS production were achieved in the medium containing Ashbya gossypii extract. Also, among various carbon sources, EPS production and the growth of A. pullulans was the best on sucrose. The Plackett–-Burman design was used to evaluate the effects of 7 variables selected from preliminary studies, and three statistically significant variables namely, sucrose, NaNO3, and Ashyba gossiipii extract, were selected.
Then, a three-level Box-Behnken design was employed to optimize the medium composition for EPS production in shake-flask. Using this methodology, the quadratic regression model of producing EPS was built and the optimal combinations of media constituents and culture conditions for maximum EPS production (about 30g/L) were determined.