earticle

영어

Proteins composed of twenty essential canonical amino acids play important biological roles in a cell and their biological activities. Recently, great efforts have been devoted to expanding the genetic code for the in vivo biological incorporation of non-canonical amino acids into proteins. The biological approaches for the incorporation of unnatural amino acids into target proteins in vivo may give us more dimensions to engineer the physico-chemical properties of proteins which are generally limited by canonical amino acids. Frequently, two experimental approaches were used for reassignment of sense (residue specific incorporation) and non sense codon (site specific incorporation) with UAA through in vivo expression of recombinant proteins. Even though, these methods successfully alter the protein functionality, the limitations of above methods is that they allow only incorporation of a single unnatural amino acid into the recombinant protein. Taking this into consideration the importance and limitation of above methods as well as giving indispensable creation of multifunctional protein, we
developed a promising alternative approach through coupling of both sense and suppression method in single protein. Herein, we report novel and facile method for incorporation of two UAA into single protein. This combination will offer extraordinary opportunity for protein engineers to create protein with novel functionality which expand the proteome of the cell.
This approach will drive us toward a post proteomics era as well as it will also open a new door for synthetic biologist to generate multi characteristic proteins