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영어

Haematococcus pluvialis is an unicellualr photosynthetic green microalgae and it produce a large amount astaxanthin under various environmental conditions such as light intensity, nitrogen deficiency and so on. In this study, we investigated that their growth and astaxanthin accumulation on red, blue and white light using LED. The growth of H. pluvialis was carried out at 25 ℃, 0.5 vvm, pH 7±0.5, light intensity from 60 μmol․m-2․s-1 to 120 μmol․m-2․s-1 on the erlenmeyer flasks. Further experiment was performed for the growth and astaxanthin accumulation in the 20 L plate photobioreactor on the red and blue light. Astaxanthin accumulation was performed at 25 ℃, 0.5 vvm, pH 7±0.5 and light intensity of 250 μmol․m-2․s-1. From the growth to the astaxanthin, light source was changed red to bule light and also light intensity was gradually increased by lumostatic method. As our results, the maximum cell numbers was 8.5×105 cells․ml-1 and the maximum astaxanthin concentration was 35 mg․L-1 on the blue LED. Astaxnathin concentration (from 2.7% to 3.3%) in our experiment was similar to the literature results (3%). Therefore, blue LED was the most efficient light source for mass cultivation and astaxanthin formulation.