earticle

영어

For the past decades, the Genetic code engineering has dramatically expanded it scope to design proteins with novel functional and biophysical property. Genetic code engineering is a recent developing method that helps to design and engineer proteins with additional chemical moiety through incorporation of non canonical amino acids. To date, several groups successfully incorporated more than 50 unnatural amino acids into recombinant proteins through invivo and invitro approach. Each report demonstrates the different characteristic functionality of unnatural amino acids and its application when incorporated into a protein. Even though, a lot of characteristic features of unnatural amino acid were reported, we are the first to demonstrate development novel fluorescent based copper biosensor through unnatural amino acid mutagenesis. In this study we show the incorporation of unnatural amino acid, L-DOPA into Green fluorescent protein render the protein metal binding property especially copper sensing property. The Copper binding property of the engineered protein was characterized by the combination of fluorescent spectroscopy, absorption spectroscopy and circular dichroism. Overall this study demonstrates that the development of selective, sensitive and robust fluorescent based metal biosensor using unnatural amino acid mutagenesis