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영어
Background. Surface camouflage using poly(ethylene glycol) (PEG) onto islet is promising approach for immunoprotection of transplanted islets. Furthermore go one step, here, we analyzed that 6-arm-PEG-catechol grafting on the islets plus low dose of FK506 are effectively improve the islets survival time under the strong rat to mouse xengenic islet transplantation model. Methods. After end group of 6-armed PEG was modified with catechol, it was grafted onto the surface of rat pancreatic islet. Then surface coverage, viability and functionality of islets were evaluated in vitro. The function of surface modified islets was also evaluated after xenotransplantation into diabetic mice. In addition, its synergistic effect with low dose of FK506 immunosuppressant was evaluated in vivo. Results.
6-arm-PEG-catechol did not affect the viability of islets for 1hr at 1% condition. The median survival time (MST) of 6-arm-PEG-catechol grafted islets was 12 days. However, the MST of unmodified islets was 10.5 days. Furthermore, the MST of 6-arm-PEG-catechol grafted islet plus 0.2 mg/kg of FK506 treatment was significantly increased compared to that of unmodified islets plus 0.2 mg/kg of FK506 treatment.(6 arm-PEG-catechol grafted islet receiving group: 21 days, unmodified islets receiving group: 10 days) Conclusion. Newly developed protocol using polymer and clinically used immunosuppressive drug would certainly be an effective combination therapy for the treatment of type 1 diabetes.