earticle

영어

In this study, we developed a novel protein-free medium for CHO DG44 host cells in suspension culture. Chinese hamster ovary (CHO) DG44 host cell line is mainly used in the production of monoclonal antibody (mAB) for therapeutic use on the market. Serum has been used in culture of recombinant CHO cells for mAB production, but since serum possesses potential contaminants, e.g. viruses, mycoplasma or prions, commercial protein-free media with serum eliminated are mostly used in mAB production. The composition of commercial media is unknown and the suspension culture of rCHO DG44 cells with commercial media requires long term adaptation periods. Development of an in-house protein-free medium with stable cell growth at adaptation-free or short term adaptation for CHO DG44 host cells will enable to overcome these limitations. We have established the formulation of protein-free components such as amino acids, iron supplement, hydrolysate, energy source, inorganic salts, etc. The maximum cell density of the medium was 7.5*106 cells/ml in batch culture with 52% higher cell growth than the commercial media.