earticle

영어

The PDA vesicles-based sensors have been interest due to their unique chromatic properties, such as blue-to-red color transition by external perturbations like pH, heat, physical stresses.1-2 Moreover, the PDA vesicles simultaneously emit the red fluorescence while changing blue to red phase. Polydiacetylene (PDA)vesicles have been demonstrated as many different types of label-free biosensors by the bichromic property. The PDA-based sensors have successfully detected various biological samples, including choleratoxin, Escherichia coli, oligonucleotides, and lipopolysaccharides since influenza virus were first detected.3-4 The liposome-like PDA vesicles are possible to have different functional groups, such as amine, carboxylic acid, and biotin, etc, by using various PDA derivatives. Therefore, the label-free PDA biosensor on the solid substrate is easily possible to be constructed.
Here, we report the microarrayed PDA-based biosensors with antibody or antigen-specific aptamer to detect bacteria cells using the stress-induced fluorescence emission of PDA vesicles. Through the results, we expect that the system of micro-patterned PDA-based biosensor immobilized on the solid substrate can be applied to make a novel label-free diagnostic sensor.