원문정보
초록
영어
The changes in bacterial communities associated with volatile fatty acids (VFAs) profiles were investigated in anaerobic batch reactors treating swine wastewater. Denaturing gradient gel electrophoresis (DGGE) and real-time QPCR were used to analyze the bacterial community structure and the methanogenic population, respectively. The three subspecies of Lactobacillus delbrueckii (bulgaricus, lactis, and indicus)along with the Clostridium-like organismswere identified.The L. delbrueckii-related organisms were assumed to be responsible for the fermentation of the organic compounds throughout the overall reaction period. The presence of C. ultunense and C. kluyveri, together with hydrogenotrophic methanogens, was essential for methane production from propionic acid in the later stage of the experiment.
The order Methanomicrobiales, hydrogenotrophic methangoens, increased rapidly as the process proceeded, resulting in its dominance above 64% after day 28, among the methanogenic groups monitored. Our results showed that the variations in the VFA concentrations and methane production were complicated and dynamic. It was also demonstrated that analyzing acidogenic community along with methanogenic population was essential for better insights into understanding anaerobic digestion of swine wastewater.