earticle

영어

The goal of this research is to develop recombinant E. coli for improved fatty acid synthesis (FAS). Genes for acetyl-CoA carboxylase (accA, accB, accC), malonyl-CoA-[acyl-carrier-protein] transacylase (fabD), and acyl-acyl carrier protein thioesterase (E3.1.2.14), which are the enzymes that catalyze the key steps in the synthesis of fatty acids, were cloned and over-expressed in E. coli MG1655. The enzymes of acc family genes catalyze the addition of CO2 to acetyl-CoA to generate malonyl-CoA, enzyme of fabD gene converts malonyl-CoA to malonyl-[acp], and gene for E3.1.2.14 converts fatty acyl-ACP chain to long chain fatty acids. All the genes were identified as homologous gene of E. coli and used to improve enzyme activities for overflowing of FAS pathway through a metabolic engineering. Eleven different E. coli MG1655 strains containing various combination of genes were developed by using pTrc99A expression vector. To observe changes in metabolism, in vivo and in vitro metabolites and fatty acids were analyzed from recombinants. The recombinant strains produced more malonic acid and fatty acids than its wild type did.