earticle

영어

To immobilize the effective and specific enzymes on nanomaterials such as nanoparticles, nanofiber and nanotube for stabilizing the activity of free enzymes has developed the various fields. In this results, Lysosomal enzymes isolated from hen`s egg white and sacharomyces cerevisiae were directly immobilized on titanium (IV) oxide (TiO2) particles using shaking methods (150 rpm, room temperature, 10 min), and the immobilization efficiency, antimicrobial activity using viable cell counts against Escherichia coli, and stability of lysosomal enzymes immobilized on TiO2 were evaluated. In addition, enhanced immobilization efficiency was shown in TiO2 pretreated with a divalent, positively charged ion, Ca2+, and the antimicrobial activity for E. coli increased as a function of increasing ratio of immobilized enzymes. Furthermore, the degradation of melanin for a week and by measuring contents of peroxidase in immobilized lysosomal enzyme, we could determine the decrease of melanin in lysosomal enzymes immobilized on TiO2 after examining the effect of different pH of solvent on the immobilization of lysosomal enzymes extracted from S. cerevisiae and egg white. We found that not only the immobilization efficiency but also other results of immobilized lysosomal enzymes extracted from S. cerevisiae were higher than those extracted from hen’s egg white with pH 4.
Therefore, Our results showed that the various activities of lysosomal enzymes immobilized on TiO2 are facilitated by sources and the pH differences.